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Time：2017-06-16Publisher：Xingyuan

Colloidal gold labeling technology is another important labeling technique after fluorescence, radioactive isotopes, and enzyme labeling techniques. Since Faulk and Taylor in 1971& Nbsp; Since the introduction of colloidal gold labeling technology into immunohistochemistry, colloidal gold labeling technology has developed and is widely used in biomedical and clinical diagnosis due to its unique characteristics that other labeling techniques do not have. In recent years, colloidal gold immunodiagnostic technology developed on the basis of colloidal gold labeling technology has become one of the sensitive immune detection technologies with broad application prospects. The colloidal gold immunodiagnostic technology developed based on enzyme-linked immunosorbent assay, latex agglutination assay, monoclonal antibody technology, chromatography technology, colloidal gold technology, and new material technology mainly includes two types in current medical testing: dot immunogold filtration method and colloidal gold immunochromatography method. And

Gold Label Card

Colloidal gold immunochromatography technology has the characteristics of simple operation, no need for technical training for operators, no need for instruments and equipment, stable reagents, and easy storage, and has become one of the main methods for" Point of Care Testing ".

Colloidal gold refers to the aggregation of gold particles of different sizes by chloroauric acid (HAuCl4) under the action of a reducing agent, forming a negatively charged hydrophobic gel solution. Due to electrostatic interaction, a stable colloidal state is formed. The dispersed phase (gold particles) in the colloidal gold solution is 1-150nm in size, belonging to a multiphase non-uniform system, and the colloidal color changes from orange red to purple.

Red transition. Colloidal gold particles consist of a basic gold nucleus (atomic gold) It is composed of a double ion layer surrounding the periphery, with an inner layer of AuCl2- negative ion layer and an outer layer of H+dispersed in the solution, which generates electrostatic attraction to maintain the stable state of the colloid. Preparation of Colloidal Gold& Nbsp; The commonly used method is chemical reduction, and commonly used reducing agents include trisodium citrate, succinic acid, ascorbic acid, thiocyanate, etc. According to the type of reducing agent and the strength of the reducing effect, colloidal gold of 0.8~150nm can be controlled to be produced. The larger the amount of reducing agent used, the smaller the diameter of the gold particles produced

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