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Time：2017-06-24Publisher：Xingyuan

Streptococcus suis has strong pathogenicity and can cause meningitis, endocarditis, sepsis, pneumonia, and even sudden death in pigs. It can also infect humans, causing sepsis, toxic shock syndrome, and meningitis. Because this disease is widely prevalent, with a large number of patients and pigs, it poses a threat to public health and the breeding industry. Ju Ying et al. established a colloidal gold immunochromatography method for detecting Streptococcus suis type 2: colloidal gold was used to label the polyclonal antibody against Streptococcus suis type 2, and colloidal gold immunochromatography was prepared=“ http://www.jpkjfz.com.cn "Target=" _self ">Gold Label Card

Test strip. The results showed that the developed colloidal gold immunochromatographic gold label card test strip had a low detection limit of 1 million CFU/mL, simple sample processing, and results could be obtained in 10 minutes. In terms of specificity, it only had a cross reaction with SS1/2, and no cross reaction with 15 groups of other common pathogenic bacteria and streptococcal genera. Moreover, its specificity and sensitivity were not changed through the detection of simulated samples. Liu Honggui et al. established a new method for detecting Staphylococcus aureus: double antibody sandwich method. This method uses colloidal gold labeling." Anti Staphylococcus aureus agglutination factor A recombinant protein specific antibody, then coated with anti Staphylococcus aureus IgG on nitrocellulose membrane to prepare an immunochromatographic test strip. The results showed that the sensitivity was 10000 CFU/mL, but it was one order of magnitude lower than the PCR method. It does not cross with Escherichia coli, Streptococcus, Listeria, Pasteurella, and Bacillus, and has good specificity

Bovine tuberculosis is a chronic consumptive infectious disease caused by Mycobacterium, and the detection method recommended by the World Health Organization is tuberculin intradermal allergy. This detection method is prone to false positive, that is, susceptible to interference from non pathogenic Mycobacterium. Ma Xianghui et al. established a colloidal gold immunochromatographic diagnostic method for bovine tuberculosis, using rabbit anti bovine serum protein and Mycobacterium bovis MPB70-83-ESAT-6 protein expressed in Escherichia coli as colloidal gold labeled antigens and capture antigens on the detection line, respectively, to prepare bovine tuberculosis antibody detection test strips. The results showed that the developed test strip did not interact with positive serum of bovine paratuberculosis, bovine Babesiosis, foot-and-mouth disease, and bovine infectious rhinotracheitis, therefore it has good specificity. In addition, the compliance rate between the gold standard cardboard test strip and TST is 80%& Nbsp

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